Download Mendeley2-Se-T-modified-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant
Fang, Z., Yang, F., Huang, Z.To be published.
5W7O | pdb_00005w7o
2-Se-T4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant
- PDB DOI: https://doi.org/10.2210/pdb5W7O/pdb
- NAKB: 5W7O
- Classification: HYDROLASE/DNA/RNA
- Organism(s): synthetic construct, Halalkalibacterium halodurans C-125
- Expression System: Escherichia coli K-12
- Mutation(s): Yes
- Deposited: 2017-06-20 Released: 2017-06-28
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.75 Å
- R-Value Free: 0.229 (Depositor), 0.240 (DCC)
- R-Value Work: 0.182 (Depositor), 0.190 (DCC)
- R-Value Observed: 0.184 (Depositor)
Starting Model: experimental
View more details
wwPDB Validation 3D Report Full Report
This is version 1.1 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 3 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Ribonuclease H | C [auth A] | 132 | Halalkalibacterium halodurans C-125 | Mutation(s): 1 Gene Names: rnhA, BH0863 EC: 3.1.26.4 |  |
UniProt | |||||
Find proteins for Q9KEI9 (Halalkalibacterium halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)) Explore Q9KEI9 Go to UniProtKB: Q9KEI9 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q9KEI9 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| MG Query on MG | D [auth A], E [auth A] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.75 Å
- R-Value Free: 0.229 (Depositor), 0.240 (DCC)
- R-Value Work: 0.182 (Depositor), 0.190 (DCC)
- R-Value Observed: 0.184 (Depositor)
Space Group: C 1 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 80.71 | α = 90 |
| b = 37.579 | β = 96.01 |
| c = 62.319 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| HKL-2000 | data reduction |
| HKL-2000 | data scaling |
| PHASER | phasing |
Entry History
Revision History (Full details and data files)
- Version 1.0: 2017-06-28
Type: Initial release - Version 1.1: 2023-10-04
Changes: Data collection, Database references, Derived calculations, Refinement description
