Domain Annotation: ECOD Classification ECOD Database Homepage

ChainsFamily NameDomain Identifier ArchitecturePossible HomologyHomologyTopologyFamilyProvenance Source (Version)
BEP_SAG101e7xeyB1 A: alpha arraysX: EDS1-PAD4 (EP) domain (From Topology)H: EDS1-PAD4 (EP) domain (From Topology)T: EDS1-PAD4 (EP) domainF: EP_SAG101ECOD (1.6)
BLipase_3e7xeyB2 A: a/b three-layered sandwichesX: alpha/beta-Hydrolases (From Topology)H: alpha/beta-Hydrolases (From Topology)T: alpha/beta-HydrolasesF: Lipase_3ECOD (1.6)
AEP_SAG101e7xeyA2 A: alpha arraysX: EDS1-PAD4 (EP) domain (From Topology)H: EDS1-PAD4 (EP) domain (From Topology)T: EDS1-PAD4 (EP) domainF: EP_SAG101ECOD (1.6)
ALipase_3e7xeyA1 A: a/b three-layered sandwichesX: alpha/beta-Hydrolases (From Topology)H: alpha/beta-Hydrolases (From Topology)T: alpha/beta-HydrolasesF: Lipase_3ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

ChainsAccessionNameDescriptionCommentsSource
PF18117Enhanced disease susceptibility 1 protein EP domain (EDS1_EP)Enhanced disease susceptibility 1 protein EP domainThe plant specific protein family, which comprises EDS1 (Enhanced disease susceptibility 1), PAD4 (Phytoalexin deficient4) and SAG101 (Senescence-associated gene 101), is involved in innate immunity [Ref.1]. Signaling by intracellular immune receptor ...The plant specific protein family, which comprises EDS1 (Enhanced disease susceptibility 1), PAD4 (Phytoalexin deficient4) and SAG101 (Senescence-associated gene 101), is involved in innate immunity [Ref.1]. Signaling by intracellular immune receptors with NB-ARC domain (Pfam:PF00931) relies on this protein family [1,3]. C-terminus of Arabidopsis EDS1 and PAD4 proteins did not have recognizable sequence homology to other domains and therefore named EP domain (EDS1-PAD4) [2]. A combination of an N-terminal lipase_3 domain (Pfam:PF01764) and the C-terminal EP-domain is the defining feature of this family. Structurally, EP domain comprises exclusively of alpha-helices (PDB:4NFU) [1]. Structure-function analysis of Arabidopsis proteins showed that EDS1 forms heterodimers with PAD4 or SAG101, chiefly through the N-terminal lipase_3 domain. These heterodimers are essential for plant defense signaling. The EP domain is not stable without the lipase_3 domain, but is required for immunity [1]. Mutations of conserved residues in the EP domain of EDS1 strongly affect its immune signaling function [4].
Domain
PF01764Lipase (class 3) (Lipase_3)Lipase (class 3)- Family
PF18117Enhanced disease susceptibility 1 protein EP domain (EDS1_EP)Enhanced disease susceptibility 1 protein EP domainThe plant specific protein family, which comprises EDS1 (Enhanced disease susceptibility 1), PAD4 (Phytoalexin deficient4) and SAG101 (Senescence-associated gene 101), is involved in innate immunity [Ref.1]. Signaling by intracellular immune receptor ...The plant specific protein family, which comprises EDS1 (Enhanced disease susceptibility 1), PAD4 (Phytoalexin deficient4) and SAG101 (Senescence-associated gene 101), is involved in innate immunity [Ref.1]. Signaling by intracellular immune receptors with NB-ARC domain (Pfam:PF00931) relies on this protein family [1,3]. C-terminus of Arabidopsis EDS1 and PAD4 proteins did not have recognizable sequence homology to other domains and therefore named EP domain (EDS1-PAD4) [2]. A combination of an N-terminal lipase_3 domain (Pfam:PF01764) and the C-terminal EP-domain is the defining feature of this family. Structurally, EP domain comprises exclusively of alpha-helices (PDB:4NFU) [1]. Structure-function analysis of Arabidopsis proteins showed that EDS1 forms heterodimers with PAD4 or SAG101, chiefly through the N-terminal lipase_3 domain. These heterodimers are essential for plant defense signaling. The EP domain is not stable without the lipase_3 domain, but is required for immunity [1]. Mutations of conserved residues in the EP domain of EDS1 strongly affect its immune signaling function [4].
Domain
PF01764Lipase (class 3) (Lipase_3)Lipase (class 3)- Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

ChainsPolymerMolecular FunctionBiological ProcessCellular Component
Lipase-like PAD4
Protein EDS1