7KRA

Cryo-EM structure of Saccharomyces cerevisiae ER membrane protein complex bound to Fab-DH4 in lipid nanodiscs

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
IMGT Antibody Annotation
SAbDab Antibody Annotation
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: MemProtMD
Membrane Protein Annotation: mpstruc

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
J	C1-set_4	e7kraJ1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_4	ECOD (1.6)
J	V-set	e7kraJ2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set	ECOD (1.6)
I	C1-set_1	e7kraI1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_1	ECOD (1.6)
I	V-set_12	e7kraI2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set_12	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	1.25.40.10	Mainly Alpha	Alpha Horseshoe	Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat	Tetratricopeptide repeat domain	CATH (utative)
I	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (utative)

IMGT Antibody Annotation IMGT Database Homepage

Chain	Protein Name	Description	Organism Name	Gene Allele Name(s)	Domain Name(s)	Receptor Type	Receptor Description	Provenance Source
J	Fab DH4	L-KAPPA	Homo sapiens (human)	IGKV2-2801, IGKV2D-2801, IGKJ401, IGKJ402, IGKC*01	V-DOMAIN V-KAPPA, C-DOMAIN C-KAPPA	IG	FAB-GAMMA-1_KAPPA	IMGT (202501-0)
I	Fab DH4	VH-CH1	Homo sapiens (human)	IGHV4-3401, IGHV4-3402, IGHJ101, IGHG101, IGHG102, IGHG104, IGHG105, IGHG112, IGHG114, IGHG113, IGHG109, IGHG110, IGHG107, IGHG111	V-DOMAIN VH, C-DOMAIN CH1	IG	FAB-GAMMA-1_KAPPA	IMGT (202501-0)

SAbDab Antibody Annotation SAbDab Database Homepage

Chain	Chain Class	Chain Subclass	Chain Type	Antigen Name(s)	Provenance Source
J	Light Chain	IGKV2	Kappa	er membrane protein complex subunit 1	SAbDab (2025-01-17)
I	Heavy Chain	IGHV4	-	er membrane protein complex subunit 1	SAbDab (2025-01-17)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF07774	ER membrane protein complex subunit 1, C-terminal (EMC1_C)	ER membrane protein complex subunit 1, C-terminal	This is the C-terminal domain of ER membrane protein complex subunit 1. ER membrane protein complex subunit 1 is a component of the ER membrane protein complex (EMC, composed of EMC1, EMC2, EMC3, EMC4, EMC5 and EMC6). In Saccharomyces cerevisiae, the ...	This is the C-terminal domain of ER membrane protein complex subunit 1. ER membrane protein complex subunit 1 is a component of the ER membrane protein complex (EMC, composed of EMC1, EMC2, EMC3, EMC4, EMC5 and EMC6). In Saccharomyces cerevisiae, the EMC seems to be required for efficient folding of proteins in the endoplasmic reticulum (ER) [1].	Domain
E	PF10270	Membrane magnesium transporter (MMgT)	Membrane magnesium transporter	-	Family
F	PF07019	EMC6 (EMC6)	EMC6	-	Family
G	PF17081	Suppressor of PMA 1-7 protein (SOP4)	Suppressor of PMA 1-7 protein	-	Family
H	PF21203	ER membrane protein complex subunit 10 (ECM10)	ER membrane protein complex subunit 10	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	ER membrane protein complex subunit 1	-	localization transport macromolecule localization phospholipid transport lipid localization lipid transport establishment of localization organophosphate ester transport primary metabolic process gene expression macromolecule biosynthetic process protein maturation protein folding biosynthetic process localization transport macromolecule localization phospholipid transport lipid localization lipid transport establishment of localization organophosphate ester transport primary metabolic process gene expression macromolecule biosynthetic process protein maturation protein folding biosynthetic process metabolic process cellular process protein metabolic process macromolecule metabolic process protein folding in endoplasmic reticulum phospholipid metabolic process organophosphate metabolic process phosphate-containing compound metabolic process phosphorus metabolic process lipid metabolic process protein localization membrane organization cellular localization establishment of protein localization establishment of protein localization to membrane protein insertion into ER membrane by stop-transfer membrane-anchor sequence localization within membrane protein localization to membrane endoplasmic reticulum organization cellular component organization organelle organization protein localization to organelle cellular macromolecule localization cellular component organization or biogenesis protein insertion into membrane protein insertion into ER membrane endomembrane system organization	membrane-bounded organelle protein-containing complex intracellular anatomical structure EMC complex organelle subcompartment membrane membrane protein complex intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle cellular anatomical structure organelle nuclear outer membrane-endoplasmic reticulum membrane network membrane-bounded organelle protein-containing complex intracellular anatomical structure EMC complex organelle subcompartment membrane membrane protein complex intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle cellular anatomical structure organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane
J	Fab DH4 light chain	-	-	-
K [auth M], L [auth N]	Unassigned helix	-	-	-
B	ER membrane protein complex subunit 2	-	localization transport macromolecule localization phospholipid transport lipid localization lipid transport establishment of localization organophosphate ester transport primary metabolic process gene expression macromolecule biosynthetic process protein maturation protein folding biosynthetic process localization transport macromolecule localization phospholipid transport lipid localization lipid transport establishment of localization organophosphate ester transport primary metabolic process gene expression macromolecule biosynthetic process protein maturation protein folding biosynthetic process metabolic process cellular process protein metabolic process macromolecule metabolic process protein folding in endoplasmic reticulum phospholipid metabolic process organophosphate metabolic process phosphate-containing compound metabolic process phosphorus metabolic process lipid metabolic process protein localization membrane organization cellular localization establishment of protein localization establishment of protein localization to membrane protein insertion into ER membrane by stop-transfer membrane-anchor sequence localization within membrane protein localization to membrane endoplasmic reticulum organization cellular component organization organelle organization protein localization to organelle cellular macromolecule localization cellular component organization or biogenesis protein insertion into membrane protein insertion into ER membrane endomembrane system organization	membrane-bounded organelle protein-containing complex intracellular anatomical structure EMC complex organelle subcompartment membrane membrane protein complex intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle cellular anatomical structure organelle nuclear outer membrane-endoplasmic reticulum membrane network membrane-bounded organelle protein-containing complex intracellular anatomical structure EMC complex organelle subcompartment membrane membrane protein complex intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle cellular anatomical structure organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane nucleus
C	ER membrane protein complex subunit 3	protein binding binding protein-folding chaperone binding	localization transport macromolecule localization phospholipid transport lipid localization lipid transport establishment of localization organophosphate ester transport primary metabolic process gene expression macromolecule biosynthetic process protein maturation protein folding biosynthetic process localization transport macromolecule localization phospholipid transport lipid localization lipid transport establishment of localization organophosphate ester transport primary metabolic process gene expression macromolecule biosynthetic process protein maturation protein folding biosynthetic process metabolic process cellular process protein metabolic process macromolecule metabolic process protein folding in endoplasmic reticulum phospholipid metabolic process organophosphate metabolic process phosphate-containing compound metabolic process phosphorus metabolic process lipid metabolic process protein localization membrane organization cellular localization establishment of protein localization establishment of protein localization to membrane protein insertion into ER membrane by stop-transfer membrane-anchor sequence localization within membrane protein localization to membrane endoplasmic reticulum organization cellular component organization organelle organization protein localization to organelle cellular macromolecule localization cellular component organization or biogenesis protein insertion into membrane protein insertion into ER membrane endomembrane system organization	membrane-bounded organelle protein-containing complex intracellular anatomical structure EMC complex organelle subcompartment membrane membrane protein complex intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle cellular anatomical structure organelle nuclear outer membrane-endoplasmic reticulum membrane network membrane-bounded organelle protein-containing complex intracellular anatomical structure EMC complex organelle subcompartment membrane membrane protein complex intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle cellular anatomical structure organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane
D	ER membrane protein complex subunit 4	-	localization transport macromolecule localization phospholipid transport lipid localization lipid transport establishment of localization organophosphate ester transport primary metabolic process gene expression macromolecule biosynthetic process protein maturation protein folding biosynthetic process localization transport macromolecule localization phospholipid transport lipid localization lipid transport establishment of localization organophosphate ester transport primary metabolic process gene expression macromolecule biosynthetic process protein maturation protein folding biosynthetic process metabolic process cellular process protein metabolic process macromolecule metabolic process protein folding in endoplasmic reticulum phospholipid metabolic process organophosphate metabolic process phosphate-containing compound metabolic process phosphorus metabolic process lipid metabolic process protein localization membrane organization cellular localization establishment of protein localization establishment of protein localization to membrane protein insertion into ER membrane by stop-transfer membrane-anchor sequence localization within membrane protein localization to membrane endoplasmic reticulum organization cellular component organization organelle organization protein localization to organelle cellular macromolecule localization cellular component organization or biogenesis protein insertion into membrane protein insertion into ER membrane endomembrane system organization	membrane-bounded organelle protein-containing complex intracellular anatomical structure EMC complex organelle subcompartment membrane membrane protein complex intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle cellular anatomical structure organelle nuclear outer membrane-endoplasmic reticulum membrane network membrane-bounded organelle protein-containing complex intracellular anatomical structure EMC complex organelle subcompartment membrane membrane protein complex intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle cellular anatomical structure organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane
E	ER membrane protein complex subunit 5	-	localization transport macromolecule localization phospholipid transport lipid localization lipid transport establishment of localization organophosphate ester transport primary metabolic process gene expression macromolecule biosynthetic process protein maturation protein folding biosynthetic process localization transport macromolecule localization phospholipid transport lipid localization lipid transport establishment of localization organophosphate ester transport primary metabolic process gene expression macromolecule biosynthetic process protein maturation protein folding biosynthetic process metabolic process cellular process protein metabolic process macromolecule metabolic process protein folding in endoplasmic reticulum phospholipid metabolic process organophosphate metabolic process phosphate-containing compound metabolic process phosphorus metabolic process lipid metabolic process protein localization membrane organization cellular localization establishment of protein localization establishment of protein localization to membrane protein insertion into ER membrane by stop-transfer membrane-anchor sequence localization within membrane protein localization to membrane endoplasmic reticulum organization cellular component organization organelle organization protein localization to organelle cellular macromolecule localization cellular component organization or biogenesis protein insertion into membrane protein insertion into ER membrane endomembrane system organization	membrane-bounded organelle protein-containing complex intracellular anatomical structure EMC complex organelle subcompartment membrane membrane protein complex intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle cellular anatomical structure organelle nuclear outer membrane-endoplasmic reticulum membrane network membrane-bounded organelle protein-containing complex intracellular anatomical structure EMC complex organelle subcompartment membrane membrane protein complex intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle cellular anatomical structure organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane
F	ER membrane protein complex subunit 6	-	localization transport macromolecule localization phospholipid transport lipid localization lipid transport establishment of localization organophosphate ester transport primary metabolic process gene expression macromolecule biosynthetic process protein maturation protein folding biosynthetic process localization transport macromolecule localization phospholipid transport lipid localization lipid transport establishment of localization organophosphate ester transport primary metabolic process gene expression macromolecule biosynthetic process protein maturation protein folding biosynthetic process metabolic process cellular process protein metabolic process macromolecule metabolic process protein folding in endoplasmic reticulum phospholipid metabolic process organophosphate metabolic process phosphate-containing compound metabolic process phosphorus metabolic process lipid metabolic process protein localization membrane organization cellular localization establishment of protein localization establishment of protein localization to membrane protein insertion into ER membrane by stop-transfer membrane-anchor sequence localization within membrane protein localization to membrane endoplasmic reticulum organization cellular component organization organelle organization protein localization to organelle cellular macromolecule localization cellular component organization or biogenesis protein insertion into membrane protein insertion into ER membrane endomembrane system organization autophagosome assembly catabolic process organelle assembly vacuole organization macroautophagy autophagy autophagosome organization cellular component biogenesis cellular component assembly process utilizing autophagic mechanism	membrane-bounded organelle protein-containing complex intracellular anatomical structure EMC complex organelle subcompartment membrane membrane protein complex intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle cellular anatomical structure organelle nuclear outer membrane-endoplasmic reticulum membrane network membrane-bounded organelle protein-containing complex intracellular anatomical structure EMC complex organelle subcompartment membrane membrane protein complex intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle cellular anatomical structure organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane
G	Protein SOP4	-	localization cellular localization transport establishment of localization in cell intracellular transport cellular process endoplasmic reticulum to Golgi vesicle-mediated transport establishment of localization Golgi vesicle transport vesicle-mediated transport protein localization establishment of protein localization nitrogen compound transport protein transport localization cellular localization transport establishment of localization in cell intracellular transport cellular process endoplasmic reticulum to Golgi vesicle-mediated transport establishment of localization Golgi vesicle transport vesicle-mediated transport protein localization establishment of protein localization nitrogen compound transport protein transport cellular macromolecule localization macromolecule localization	cellular anatomical structure membrane membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle endoplasmic reticulum cytoplasm intracellular membrane-bounded organelle endomembrane system organelle subcompartment endoplasmic reticulum subcompartment nuclear outer membrane-endoplasmic reticulum membrane network organelle membrane cellular anatomical structure membrane membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle endoplasmic reticulum cytoplasm intracellular membrane-bounded organelle endomembrane system organelle subcompartment endoplasmic reticulum subcompartment nuclear outer membrane-endoplasmic reticulum membrane network organelle membrane endoplasmic reticulum membrane
H	Endoplasmic reticulum membrane protein complex subunit 10	-	protein localization membrane organization cellular localization establishment of protein localization establishment of protein localization to membrane protein insertion into ER membrane by stop-transfer membrane-anchor sequence localization within membrane protein localization to membrane endoplasmic reticulum organization establishment of localization cellular component organization localization organelle organization protein localization to organelle cellular macromolecule localization protein localization membrane organization cellular localization establishment of protein localization establishment of protein localization to membrane protein insertion into ER membrane by stop-transfer membrane-anchor sequence localization within membrane protein localization to membrane endoplasmic reticulum organization establishment of localization cellular component organization localization organelle organization protein localization to organelle cellular macromolecule localization macromolecule localization cellular process cellular component organization or biogenesis protein insertion into membrane protein insertion into ER membrane endomembrane system organization	membrane-bounded organelle protein-containing complex intracellular anatomical structure EMC complex organelle subcompartment membrane membrane protein complex intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle cellular anatomical structure organelle nuclear outer membrane-endoplasmic reticulum membrane network membrane-bounded organelle protein-containing complex intracellular anatomical structure EMC complex organelle subcompartment membrane membrane protein complex intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle cellular anatomical structure organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane
I	Fab DH4 heavy chain	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR011678	ER membrane protein complex subunit 1, C-terminal	Domain
A	IPR026895	ER membrane protein complex subunit 1	Family
B	IPR039856	ER membrane protein complex subunit 2-like	Family
B	IPR011990	Tetratricopeptide-like helical domain superfamily	Homologous Superfamily
B	IPR019734	Tetratricopeptide repeat	Repeat
C	IPR008568	ER membrane protein complex subunit 3	Family
C	IPR002809	Integral membrane protein EMC3/TMCO1-like	Family
D	IPR009445	ER membrane protein complex subunit 4	Family
E	IPR018937	Membrane magnesium transporter	Family
E	IPR053279	ER membrane protein complex subunit	Family
F	IPR008504	ER membrane protein complex subunit 6	Family
F	IPR029008	ER membrane protein complex subunit 6-like	Family
G	IPR031395	Protein Sop4	Family

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B, C, D, E A, B, C, D, E, F, G, H	OPM				ER membrane protein insertion complex

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A, B, C, D, E A, B, C, D, E, F, G, H	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A, B, C, D, E A, B, C, D, E, F, G, H	MemProtMD	Annotated as membrane protein by MemProtMD

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A, B, C, D, E A, B, C, D, E, F, G, H	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	Sec, Translocase, and Insertase Proteins	ER membrane protein complex (EMC) in DDM detergent

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.