Annotations: 6P07

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Vps4_C_C	e6p07A1	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: Vps4_C_C	ECOD (1.6)
A	Sigma54_activat	e6p07A2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Sigma54_activat	ECOD (1.6)
B	Vps4_C_C	e6p07B1	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: Vps4_C_C	ECOD (1.6)
B	Sigma54_activat	e6p07B2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Sigma54_activat	ECOD (1.6)
F	Vps4_C_C	e6p07F2	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: Vps4_C_C	ECOD (1.6)
F	Sigma54_activat	e6p07F1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Sigma54_activat	ECOD (1.6)
C	Vps4_C_C	e6p07C2	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: Vps4_C_C	ECOD (1.6)
C	Sigma54_activat	e6p07C1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Sigma54_activat	ECOD (1.6)
D	Vps4_C_C	e6p07D1	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: Vps4_C_C	ECOD (1.6)
D	Sigma54_activat	e6p07D2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Sigma54_activat	ECOD (1.6)
E	Vps4_C_C	e6p07E2	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: Vps4_C_C	ECOD (1.6)
E	Sigma54_activat	e6p07E1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Sigma54_activat	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F	PF09336	Vps4 C terminal oligomerisation domain (Vps4_C)	Vps4 C terminal oligomerisation domain	This domain is found at the C terminal of ATPase proteins involved in vacuolar sorting. It forms an alpha helix structure and is required for oligomerisation [1].	Domain
A, B, C, D, E A, B, C, D, E, F	PF17862	AAA+ lid domain (AAA_lid_3)	AAA+ lid domain	This entry represents the alpha helical AAA+ lid domain that is found to the C-terminus of AAA domains.	Domain
A, B, C, D, E A, B, C, D, E, F	PF00004	ATPase family associated with various cellular activities (AAA) (AAA)	ATPase family associated with various cellular activities (AAA)	AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F	Spastin	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding cytoskeletal protein binding microtubule binding protein binding tubulin binding polypeptide conformation or assembly isomerase activity protein-containing complex destabilizing activity isomerase activity microtubule destabilizing activity microtubule severing ATPase activity catalytic activity, acting on a protein catalytic activity macromolecular conformation isomerase activity ATP-dependent activity pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides	biological regulation regulation of synapse structure or activity regulation of biological quality developmental process cellular developmental process cell differentiation cellular process cellular component organization organelle organization microtubule severing microtubule cytoskeleton organization microtubule-based process cellular component organization or biogenesis cytoskeleton organization biological regulation regulation of synapse structure or activity regulation of biological quality developmental process cellular developmental process cell differentiation cellular process cellular component organization organelle organization microtubule severing microtubule cytoskeleton organization microtubule-based process cellular component organization or biogenesis cytoskeleton organization adult locomotory behavior behavior multicellular organismal process adult behavior locomotory behavior mitotic cell cycle localization mitotic chromosome movement towards spindle pole chromosome localization organelle localization mitotic sister chromatid segregation cell cycle chromosome segregation chromosome movement towards spindle pole cell cycle process mitotic nuclear division organelle fission chromosome organization sister chromatid segregation nuclear division mitotic cell cycle process nuclear chromosome segregation microtubule-based movement cell division cellular component biogenesis protein hexamerization cellular component assembly protein-containing complex organization protein complex oligomerization protein-containing complex assembly positive regulation of organelle organization regulation of cytoskeleton organization regulation of microtubule polymerization or depolymerization regulation of cellular component organization regulation of cellular process positive regulation of cellular component organization positive regulation of biological process protein-containing complex disassembly positive regulation of microtubule depolymerization regulation of protein-containing complex disassembly microtubule polymerization or depolymerization regulation of microtubule depolymerization positive regulation of protein-containing complex disassembly positive regulation of cellular process cellular component disassembly regulation of biological process positive regulation of microtubule polymerization or depolymerization regulation of protein depolymerization positive regulation of protein depolymerization positive regulation of cytoskeleton organization regulation of microtubule-based process positive regulation of supramolecular fiber organization regulation of organelle organization microtubule depolymerization regulation of supramolecular fiber organization supramolecular fiber organization protein depolymerization regulation of microtubule cytoskeleton organization multicellular organism development anatomical structure development nervous system development system development mitotic spindle elongation spindle elongation microtubule cytoskeleton organization involved in mitosis spindle organization mitotic spindle organization	cellular anatomical structure membrane intracellular organelle centrosome microtubule organizing center intracellular anatomical structure cytoskeleton organelle membraneless organelle intracellular membraneless organelle microtubule cytoskeleton cytoplasm chromosome lipid droplet cellular anatomical structure membrane intracellular organelle centrosome microtubule organizing center intracellular anatomical structure cytoskeleton organelle membraneless organelle intracellular membraneless organelle microtubule cytoskeleton cytoplasm chromosome lipid droplet spindle polymeric cytoskeletal fiber supramolecular fiber microtubule supramolecular polymer supramolecular complex
G	polyglutamate peptide	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F	IPR017179	Spastin	Family
A, B, C, D, E A, B, C, D, E, F	IPR003593	AAA+ ATPase domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR003959	ATPase, AAA-type, core	Domain
A, B, C, D, E A, B, C, D, E, F	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR007330	MIT domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR041569	AAA ATPase, AAA+ lid domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR015415	Spastin/Vps4, C-terminal	Domain
A, B, C, D, E A, B, C, D, E, F	IPR050304	Microtubule-severing AAA ATPase	Family
A, B, C, D, E A, B, C, D, E, F	IPR003960	ATPase, AAA-type, conserved site	Conserved Site
A, B, C, D, E A, B, C, D, E, F	IPR036181	MIT domain superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.