6B44 | pdb_00006b44

Cryo-EM structure of Type I-F CRISPR crRNA-guided Csy surveillance complex with bound target dsDNA

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
I [auth L]	SCOP2B Superfamily	CRISPR-associated proteins	8052171	3001554	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	UNK_F_TYPE	e6b44A1	A: a+b complex topology	X: Cas8-like	H: Cas8f/Csy1 (From Topology)	T: Cas8f/Csy1	F: UNK_F_TYPE	ECOD (1.6)
B	UNK_F_TYPE	e6b44B1	A: a+b two layers	X: Alpha-beta plaits	H: CRISPR system Cas5 homologs (From Topology)	T: CRISPR system Cas5 homologs	F: UNK_F_TYPE	ECOD (1.6)
C	UNK_F_TYPE	e6b44C1	A: a+b two layers	X: Alpha-beta plaits	H: Cas7-related	T: CRISPR-associated protein Cas7f/Csy3	F: UNK_F_TYPE	ECOD (1.6)
D	UNK_F_TYPE	e6b44D1	A: a+b two layers	X: Alpha-beta plaits	H: Cas7-related	T: CRISPR-associated protein Cas7f/Csy3	F: UNK_F_TYPE	ECOD (1.6)
E	UNK_F_TYPE	e6b44E1	A: a+b two layers	X: Alpha-beta plaits	H: Cas7-related	T: CRISPR-associated protein Cas7f/Csy3	F: UNK_F_TYPE	ECOD (1.6)
F	UNK_F_TYPE	e6b44F1	A: a+b two layers	X: Alpha-beta plaits	H: Cas7-related	T: CRISPR-associated protein Cas7f/Csy3	F: UNK_F_TYPE	ECOD (1.6)
G	UNK_F_TYPE	e6b44G1	A: a+b two layers	X: Alpha-beta plaits	H: Cas7-related	T: CRISPR-associated protein Cas7f/Csy3	F: UNK_F_TYPE	ECOD (1.6)
H	UNK_F_TYPE	e6b44H1	A: a+b two layers	X: Alpha-beta plaits	H: Cas7-related	T: CRISPR-associated protein Cas7f/Csy3	F: UNK_F_TYPE	ECOD (1.6)
I [auth L]	Cas_Csy4_C	e6b44L2	A: a+b two layers	X: Alpha-beta plaits	H: CRISPR transcript (pre-crRNA) processing endoribonuclease-related (From Topology)	T: CRISPR transcript (pre-crRNA) processing endoribonuclease-related	F: Cas_Csy4_C	ECOD (1.6)
I [auth L]	Cas_Csy4_N	e6b44L1	A: a+b two layers	X: Alpha-beta plaits	H: CRISPR transcript (pre-crRNA) processing endoribonuclease-related (From Topology)	T: CRISPR transcript (pre-crRNA) processing endoribonuclease-related	F: Cas_Csy4_N	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
I [auth L]	3.30.70.2540	Alpha Beta	2-Layer Sandwich	Alpha-Beta Plaits	CRISPR-associated endoribonuclease Cas6/Csy4	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF09611	CRISPR-associated protein (Cas_Csy1) (Cas_Csy1)	CRISPR-associated protein (Cas_Csy1)	-	Family
B	PF09614	CRISPR-associated protein (Cas_Csy2) (Cas_Csy2)	CRISPR-associated protein (Cas_Csy2)	-	Family
C, D, E, F, G C, D, E, F, G, H	PF09615	CRISPR-associated protein (Cas_Csy3) (Cas_Csy3)	CRISPR-associated protein (Cas_Csy3)	-	Family
I [auth L]	PF09618	CRISPR-associated protein (Cas_Csy4) (Cas_Csy4)	CRISPR-associated protein (Cas_Csy4)	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	CRISPR-associated protein Csy1	-	biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response response to external stimulus response to biotic stimulus defense response to virus defense response to other organism response to stress response to external biotic stimulus response to virus	-
B	CRISPR-associated protein Csy2	-	biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response response to external stimulus response to biotic stimulus defense response to virus defense response to other organism response to stress response to external biotic stimulus response to virus	-
C, D, E, F, G C, D, E, F, G, H	CRISPR-associated protein Csy3	-	biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response response to external stimulus response to biotic stimulus defense response to virus defense response to other organism response to stress response to external biotic stimulus response to virus	-
I [auth L]	CRISPR-associated endonuclease Cas6/Csy4	endonuclease activity nuclease activity hydrolase activity, acting on ester bonds hydrolase activity catalytic activity RNA binding binding nucleic acid binding	biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response response to external stimulus response to biotic stimulus defense response to virus defense response to other organism response to stress response to external biotic stimulus response to virus maintenance of CRISPR repeat elements chromosome organization primary metabolic process biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response response to external stimulus response to biotic stimulus defense response to virus defense response to other organism response to stress response to external biotic stimulus response to virus maintenance of CRISPR repeat elements chromosome organization primary metabolic process DNA metabolic process maintenance of DNA repeat elements nucleic acid metabolic process cellular component organization nucleobase-containing compound metabolic process organelle organization cellular process metabolic process cellular component organization or biogenesis macromolecule metabolic process	-
J [auth M]	Pseudomonas aeruginosa strain SMC4485 CRISPR repeat sequence	-	-	-
K [auth N]	Target DNA strand (41-MER)	-	-	-
L [auth O]	Non-target DNA strand (5'-D(PCPAPGPTPCPAPTPCPAPCPCPAP*A)-3')	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR013397	Type I-F CRISPR-associated protein Csy1	Family
B	IPR013398	Type I-F CRISPR-associated protein Csy2	Family
C, D, E, F, G C, D, E, F, G, H	IPR013399	Type I-F CRISPR-associated protein Csy3	Family
I [auth L]	IPR013396	CRISPR-associated endoribonuclease Cas6/Csy4, subtype I-F/YPEST	Family
I [auth L]	IPR042564	CRISPR-associated endoribonuclease Cas6/Csy4, subtype I-F/YPEST superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.