5MHF

Murine endoplasmic reticulum alpha-glucosidase I with N-9'-methoxynonyl-1-deoxynojirimycin.

External Resource: Annotation

Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Protein Modification Annotation

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	Glyco_hydro_63_N	e5mhfB2	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_63_N	ECOD (1.6)
B	Glyco_hydro_63	e5mhfB1	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: Glyco_hydro_63	ECOD (1.6)
C	Glyco_hydro_63_N	e5mhfC1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_63_N	ECOD (1.6)
C	Glyco_hydro_63	e5mhfC2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: Glyco_hydro_63	ECOD (1.6)
D	Glyco_hydro_63_N	e5mhfD1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_63_N	ECOD (1.6)
D	Glyco_hydro_63	e5mhfD2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: Glyco_hydro_63	ECOD (1.6)
A	Glyco_hydro_63_N	e5mhfA1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_63_N	ECOD (1.6)
A	Glyco_hydro_63	e5mhfA2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: Glyco_hydro_63	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF03200	Glycosyl hydrolase family 63 C-terminal domain (Glyco_hydro_63)	Glycosyl hydrolase family 63 C-terminal domain	-	Repeat
A, B, C, D	PF16923	Glycosyl hydrolase family 63 N-terminal domain (Glyco_hydro_63N)	Glycosyl hydrolase family 63 N-terminal domain	This is a family of eukaryotic enzymes belonging to glycosyl hydrolase family 63. They catalyse the specific cleavage of the non-reducing terminal glucose residue from Glc(3)Man(9)GlcNAc(2). Mannosyl oligosaccharide glucosidase EC:3.2.1.106 is the f ...	This is a family of eukaryotic enzymes belonging to glycosyl hydrolase family 63. They catalyse the specific cleavage of the non-reducing terminal glucose residue from Glc(3)Man(9)GlcNAc(2). Mannosyl oligosaccharide glucosidase EC:3.2.1.106 is the first enzyme in the N-linked oligosaccharide processing pathway. This family represents the N-terminal beta sandwich domain [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Mannosyl-oligosaccharide glucosidase	alpha-glucosidase activity hydrolase activity, acting on glycosyl bonds glucosidase activity hydrolase activity, hydrolyzing O-glycosyl compounds hydrolase activity Glc3Man9GlcNAc2 oligosaccharide glucosidase activity catalytic activity	carbohydrate metabolic process oligosaccharide metabolic process primary metabolic process metabolic process cellular process	membrane-bounded organelle intracellular anatomical structure organelle subcompartment membrane intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle cellular anatomical structure organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane membrane-bounded organelle intracellular anatomical structure organelle subcompartment membrane intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle cellular anatomical structure organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane endoplasmic reticulum membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR012341	Six-hairpin glycosidase-like superfamily	Homologous Superfamily
A, B, C, D	IPR031335	Glycosyl hydrolase family 63, C-terminal	Domain
A, B, C, D	IPR004888	Glycoside hydrolase family 63	Family
A, B, C, D	IPR008928	Six-hairpin glycosidase superfamily	Homologous Superfamily
A, B, C, D	IPR038518	Glycosyl hydrolase family 63, N-terminal domain superfamily	Homologous Superfamily
A, B, C, D	IPR031631	Glycosyl hydrolase family 63, N-terminal	Domain

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
A, B, C, D	MLY	Parent Component: LYS RESID: AA0075 PSI-MOD : N6,N6-dimethyl-L-lysine MOD:00084
A, B, C, D	SME	Parent Component: MET RESID: AA0075 , AA0581 PSI-MOD : N6,N6-dimethyl-L-lysine MOD:00084 , L-methionine (S)-sulfoxide MOD:00721

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.