4EQ6 | pdb_00004eq6

The crystal structure of Psy3-Csm2 complex from budding yeast

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	RecA-like P-loop NTPases	8093037	3002019	SCOP2B (2022-06-29)
B	SCOP2 Family	Psy3-like	8046220	4004964	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	RecA-like P-loop NTPases	8093040	3002019	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	CSM2	e4eq6A1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: CSM2	ECOD (1.6)
B	PSY3	e4eq6B1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PSY3	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
B	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF16834	Shu complex component Csm2, DNA-binding (CSM2)	Shu complex component Csm2, DNA-binding	CSM2 is one of the components of the yeast Shu complex that maintains genomic stability during replication. CSM2 complexes first with Psy3, and their L2 loops confer the DNA-binding activity to the Shu complex [1,2]. The Shu complex binds to recombi ...	CSM2 is one of the components of the yeast Shu complex that maintains genomic stability during replication. CSM2 complexes first with Psy3, and their L2 loops confer the DNA-binding activity to the Shu complex [1,2]. The Shu complex binds to recombination sites and is required for Rad51 assembly and function during meiosis. The heterodimer of Psy3-Csm2 stabilises the Rad51-single-stranded DNA complex independently of nucleotide cofactor because Psy3-Csm2 is a structural mimic of the Rad51-dimer [3].	Domain
B	PF16836	Shu complex component Psy3, DNA-binding description (PSY3)	Shu complex component Psy3, DNA-binding description	PSY3 is one of the components of the yeast Shu complex that maintains genomic stability during replication. Psy3 complexes first with Cms2, and their L2 loops confer the DNA-binding activity to the Shu complex [1,2]. The Shu complex binds to recombi ...	PSY3 is one of the components of the yeast Shu complex that maintains genomic stability during replication. Psy3 complexes first with Cms2, and their L2 loops confer the DNA-binding activity to the Shu complex [1,2]. The Shu complex binds to recombination sites and is required for Rad51 assembly and function during meiosis. The heterodimer of Psy3-Csm2 stabilises the Rad51-single-stranded DNA complex independently of nucleotide cofactor because Psy3-Csm2 is a structural mimic of the Rad51-dimer [3].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Chromosome segregation in meiosis protein 2	-	cellular response to stimulus response to stimulus DNA recombination primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process cellular response to stress DNA damage response response to stress recombinational repair metabolic process cellular process macromolecule metabolic process cellular response to stimulus response to stimulus DNA recombination primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process cellular response to stress DNA damage response response to stress recombinational repair metabolic process cellular process macromolecule metabolic process DNA repair meiotic chromosome segregation sexual reproduction reproductive process meiotic cell cycle organelle fission meiotic cell cycle process cellular component organization nuclear division organelle organization nuclear chromosome segregation meiotic nuclear division cell cycle cellular component organization or biogenesis chromosome segregation cell cycle process positive regulation of single-strand break repair via homologous recombination postreplication repair regulation of DNA recombination regulation of cellular process single strand break repair regulation of cellular response to stress positive regulation of biological process positive regulation of single strand break repair regulation of primary metabolic process positive regulation of response to stimulus positive regulation of metabolic process positive regulation of DNA repair regulation of response to stress positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process positive regulation of cellular process positive regulation of DNA recombination regulation of biological process positive regulation of nucleobase-containing compound metabolic process regulation of single strand break repair biological regulation single-strand break repair via homologous recombination regulation of macromolecule metabolic process regulation of DNA repair error-free postreplication DNA repair regulation of single-strand break repair via homologous recombination positive regulation of DNA metabolic process regulation of response to stimulus regulation of metabolic process regulation of DNA metabolic process cellular component biogenesis protein-DNA complex organization DNA recombinase assembly cellular component assembly protein-containing complex assembly DNA repair complex assembly double-strand break repair via homologous recombination double-strand break repair protein-containing complex organization protein-DNA complex assembly double-strand break repair via synthesis-dependent strand annealing chromosome organization maintenance of DNA repeat elements maintenance of rDNA	protein-containing complex Shu complex cellular anatomical structure intracellular anatomical structure cytoplasm intracellular organelle site of double-strand break organelle membraneless organelle site of DNA damage intracellular membraneless organelle chromosome membrane-bounded organelle nucleus protein-containing complex Shu complex cellular anatomical structure intracellular anatomical structure cytoplasm intracellular organelle site of double-strand break organelle membraneless organelle site of DNA damage intracellular membraneless organelle chromosome membrane-bounded organelle nucleus intracellular membrane-bounded organelle
B	Platinum sensitivity protein 3	-	cellular response to stimulus response to stimulus DNA recombination primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process cellular response to stress DNA damage response response to stress recombinational repair metabolic process cellular process macromolecule metabolic process cellular response to stimulus response to stimulus DNA recombination primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process cellular response to stress DNA damage response response to stress recombinational repair metabolic process cellular process macromolecule metabolic process DNA repair positive regulation of single-strand break repair via homologous recombination postreplication repair regulation of DNA recombination regulation of cellular process single strand break repair regulation of cellular response to stress positive regulation of biological process positive regulation of single strand break repair regulation of primary metabolic process positive regulation of response to stimulus positive regulation of metabolic process positive regulation of DNA repair regulation of response to stress positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process positive regulation of cellular process positive regulation of DNA recombination regulation of biological process positive regulation of nucleobase-containing compound metabolic process regulation of single strand break repair biological regulation single-strand break repair via homologous recombination regulation of macromolecule metabolic process regulation of DNA repair error-free postreplication DNA repair regulation of single-strand break repair via homologous recombination positive regulation of DNA metabolic process regulation of response to stimulus regulation of metabolic process regulation of DNA metabolic process cellular component organization cellular component biogenesis protein-DNA complex organization DNA recombinase assembly cellular component assembly protein-containing complex assembly DNA repair complex assembly cellular component organization or biogenesis double-strand break repair via homologous recombination double-strand break repair protein-containing complex organization protein-DNA complex assembly double-strand break repair via synthesis-dependent strand annealing translesion synthesis nucleobase-containing compound biosynthetic process DNA synthesis involved in DNA repair macromolecule biosynthetic process error-free translesion synthesis nucleic acid biosynthetic process biosynthetic process DNA biosynthetic process	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol protein-containing complex Shu complex intracellular organelle site of double-strand break organelle membraneless organelle site of DNA damage intracellular membraneless organelle chromosome membrane-bounded organelle cellular anatomical structure intracellular anatomical structure cytoplasm cytosol protein-containing complex Shu complex intracellular organelle site of double-strand break organelle membraneless organelle site of DNA damage intracellular membraneless organelle chromosome membrane-bounded organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR031783	Chromosome segregation in meiosis protein 2	Family
A	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
B	IPR031779	Shu complex, component Psy3	Family
B	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.