1OUZ | pdb_00001ouz

Crystal structure of a mutant IHF (BetaE44A) complexed with a variant H' Site (T44A)

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
D [auth A]	d1ouza_	All alpha proteins	IHF-like DNA-binding proteins	IHF-like DNA-binding proteins	Prokaryotic DNA-bending protein	Integration host factor alpha subunit (IHFA)	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
E [auth B]	d1ouzb_	All alpha proteins	IHF-like DNA-binding proteins	IHF-like DNA-binding proteins	Prokaryotic DNA-bending protein	Integration host factor beta subunit (IHFB)	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
D [auth A]	SCOP2B Superfamily	IHF-like DNA-binding proteins	8034409	3000426	SCOP2B (2022-06-29)
E [auth B]	SCOP2B Superfamily	IHF-like DNA-binding proteins	8034410	3000426	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
D [auth A]	Bac_DNA_binding	e1ouzA1	A: alpha arrays	X: HTH	H: IHF-like DNA-binding proteins (From Topology)	T: IHF-like DNA-binding proteins	F: Bac_DNA_binding	ECOD (1.6)
E [auth B]	Bac_DNA_binding	e1ouzB1	A: alpha arrays	X: HTH	H: IHF-like DNA-binding proteins (From Topology)	T: IHF-like DNA-binding proteins	F: Bac_DNA_binding	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
D [auth A]	4.10.520.10	Few Secondary Structures	Irregular	HU Protein	Chain A	CATH (4.3.0)
E [auth B]	4.10.520.10	Few Secondary Structures	Irregular	HU Protein	Chain A	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
D [auth A]	PF00216	Bacterial DNA-binding protein (Bac_DNA_binding)	Bacterial DNA-binding protein		Domain
E [auth B]	PF00216	Bacterial DNA-binding protein (Bac_DNA_binding)	Bacterial DNA-binding protein		Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth C]	Phage lambda H' site	-	-	-
B [auth D]	5'-D(GPGPCPCPAPAPAPAPAPAPGPCPAPTP*T)-3'	-	-	-
C [auth E]	5'-D(GPCPTPTPAPTPCPAPAPTPTPTPGPTPAPGPCPAPCPC)-3'	-	-	-
D [auth A]	Integration Host Factor Alpha-subunit	DNA binding binding nucleic acid binding sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding double-stranded DNA binding transcription regulatory region nucleic acid binding transcription regulator activity DNA-binding transcription factor activity DNA-binding transcription activator activity structural molecule activity structural constituent of chromatin	nucleobase-containing compound metabolic process DNA replication metabolic process cellular process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process regulation of biosynthetic process regulation of biological process regulation of cellular process regulation of protein metabolic process biological regulation regulation of macromolecule metabolic process nucleobase-containing compound metabolic process DNA replication metabolic process cellular process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process regulation of biosynthetic process regulation of biological process regulation of cellular process regulation of protein metabolic process biological regulation regulation of macromolecule metabolic process gene expression translation macromolecule biosynthetic process regulation of primary metabolic process post-transcriptional regulation of gene expression regulation of gene expression biosynthetic process regulation of translation regulation of macromolecule biosynthetic process protein metabolic process regulation of metabolic process RNA metabolic process nucleobase-containing compound biosynthetic process RNA biosynthetic process DNA-templated transcription nucleic acid biosynthetic process DNA recombination	bacterial nucleoid protein-containing complex intracellular anatomical structure protein-DNA complex intracellular membraneless organelle IHF-DNA complex nucleoid DNA bending complex intracellular organelle cellular anatomical structure organelle membraneless organelle cytoplasm cytosol
E [auth B]	Integration Host Factor Beta-subunit	DNA binding binding nucleic acid binding structural molecule activity structural constituent of chromatin	nucleobase-containing compound metabolic process DNA replication metabolic process cellular process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process regulation of cellular process RNA metabolic process regulation of primary metabolic process RNA biosynthetic process nucleic acid biosynthetic process DNA-templated transcription nucleobase-containing compound metabolic process DNA replication metabolic process cellular process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process regulation of cellular process RNA metabolic process regulation of primary metabolic process RNA biosynthetic process nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of biological process biological regulation regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process regulation of gene expression biosynthetic process regulation of macromolecule biosynthetic process regulation of DNA-templated transcription regulation of metabolic process regulation of protein metabolic process translation post-transcriptional regulation of gene expression regulation of translation protein metabolic process DNA recombination	bacterial nucleoid protein-containing complex intracellular anatomical structure protein-DNA complex intracellular membraneless organelle IHF-DNA complex nucleoid DNA bending complex intracellular organelle cellular anatomical structure organelle membraneless organelle chromosome cytoplasm bacterial nucleoid protein-containing complex intracellular anatomical structure protein-DNA complex intracellular membraneless organelle IHF-DNA complex nucleoid DNA bending complex intracellular organelle cellular anatomical structure organelle membraneless organelle chromosome cytoplasm cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
D [auth A]	IPR010992	Integration host factor (IHF)-like DNA-binding domain superfamily	Homologous Superfamily
D [auth A]	IPR020816	Histone-like DNA-binding protein, conserved site	Conserved Site
D [auth A]	IPR005684	Integration host factor, alpha subunit	Family
D [auth A]	IPR000119	Histone-like DNA-binding protein	Family
E [auth B]	IPR010992	Integration host factor (IHF)-like DNA-binding domain superfamily	Homologous Superfamily
E [auth B]	IPR020816	Histone-like DNA-binding protein, conserved site	Conserved Site
E [auth B]	IPR005685	Integration host factor, beta subunit	Family
E [auth B]	IPR000119	Histone-like DNA-binding protein	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.