1MS8

Triclinic form of Trypanosoma cruzi trans-sialidase, in complex with 3-deoxy-2,3-dehydro-N-acetylneuraminic acid (DANA)

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1ms8a1	All beta proteins	Concanavalin A-like lectins/glucanases	Concanavalin A-like lectins/glucanases	Trypanosoma sialidase, C-terminal domain	Trypanosoma sialidase, C-terminal domain	(Trypanosoma cruzi ) [TaxId: 5693 ],	SCOPe (2.08)
A	d1ms8a2	All beta proteins	6-bladed beta-propeller	Sialidases	Sialidases (neuraminidases)	Trypanosoma sialidase	(Trypanosoma cruzi ) [TaxId: 5693 ],	SCOPe (2.08)
B	d1ms8b1	All beta proteins	Concanavalin A-like lectins/glucanases	Concanavalin A-like lectins/glucanases	Trypanosoma sialidase, C-terminal domain	Trypanosoma sialidase, C-terminal domain	(Trypanosoma cruzi ) [TaxId: 5693 ],	SCOPe (2.08)
B	d1ms8b2	All beta proteins	6-bladed beta-propeller	Sialidases	Sialidases (neuraminidases)	Trypanosoma sialidase	(Trypanosoma cruzi ) [TaxId: 5693 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Concanavalin A-like lectins/glucanases	8037009	3000120	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Sialidases	8037008	3001607	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Concanavalin A-like lectins/glucanases	8037009	3000120	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Sialidases	8037008	3001607	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Laminin_G_3	e1ms8A1	A: beta sandwiches	X: jelly-roll	H: Concanavalin A-like	T: Concanavalin A-like lectins/glucanases	F: Laminin_G_3	ECOD (1.6)
A	BNR_2	e1ms8A2	A: beta duplicates or obligate multimers	X: beta-propeller-like	H: beta-propeller	T: 6-bladed	F: BNR_2	ECOD (1.6)
B	Laminin_G_3	e1ms8B1	A: beta sandwiches	X: jelly-roll	H: Concanavalin A-like	T: Concanavalin A-like lectins/glucanases	F: Laminin_G_3	ECOD (1.6)
B	BNR_2	e1ms8B2	A: beta duplicates or obligate multimers	X: beta-propeller-like	H: beta-propeller	T: 6-bladed	F: BNR_2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.120.10.10	Mainly Beta	6 Propeller	Neuraminidase		CATH (4.3.0)
A	2.60.120.200	Mainly Beta	Sandwich	Jelly Rolls		CATH (4.3.0)
B	2.120.10.10	Mainly Beta	6 Propeller	Neuraminidase		CATH (4.3.0)
B	2.60.120.200	Mainly Beta	Sandwich	Jelly Rolls		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF13859	BNR repeat-like domain (BNR_3)	BNR repeat-like domain	-	Repeat
A, B	PF22925	Trans-sialidase, C-terminal domain (TS_C)	Trans-sialidase, C-terminal domain	This domain is found C-terminal in a number of Trans-sialidases (TS) from Trypanosoma cruzi and other related species. The Trypanosoma TS is involved in host cell invasion and parasite survival in the bloodstream. The parasite uses TS activity to sia ...	This domain is found C-terminal in a number of Trans-sialidases (TS) from Trypanosoma cruzi and other related species. The Trypanosoma TS is involved in host cell invasion and parasite survival in the bloodstream. The parasite uses TS activity to sialylate its own surface molecules, allowing it to evade lysis by serum factors. This domain follows the catalytic domain and adopts a beta-sandwich fold typical for Concanavalin-like lectin/glucanase superfamily. It is likely involved in binding glycan structures present on the surface of the Trypanosomes or host cells and in modulating the activity and biological function of TS [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	trans-sialidase	hydrolase activity, acting on glycosyl bonds alpha-sialidase activity hydrolase activity, hydrolyzing O-glycosyl compounds hydrolase activity exo-alpha-sialidase activity catalytic activity	membrane lipid metabolic process sphingolipid catabolic process ceramide metabolic process ganglioside catabolic process carbohydrate derivative catabolic process glycosphingolipid catabolic process catabolic process lipid catabolic process glycolipid metabolic process glycosphingolipid metabolic process liposaccharide metabolic process carbohydrate derivative metabolic process primary metabolic process sphingolipid metabolic process membrane lipid catabolic process membrane lipid metabolic process sphingolipid catabolic process ceramide metabolic process ganglioside catabolic process carbohydrate derivative catabolic process glycosphingolipid catabolic process catabolic process lipid catabolic process glycolipid metabolic process glycosphingolipid metabolic process liposaccharide metabolic process carbohydrate derivative metabolic process primary metabolic process sphingolipid metabolic process membrane lipid catabolic process glycolipid catabolic process ceramide catabolic process metabolic process cellular process amide metabolic process ganglioside metabolic process lipid metabolic process carbohydrate metabolic process oligosaccharide catabolic process oligosaccharide metabolic process carbohydrate catabolic process	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure organelle intracellular membrane-bounded organelle membrane cytoplasm

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR013320	Concanavalin A-like lectin/glucanase domain superfamily	Homologous Superfamily
A, B	IPR011040	Sialidase	Domain
A, B	IPR026856	Sialidase family	Family
A, B	IPR055239	Trans-sialidase, C-terminal domain	Domain
A, B	IPR008377	Trypanosome sialidase	Family
A, B	IPR036278	Sialidase superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.