8RSD

Thaumatin measured via serial crystallography from a kapton HARE-chip (125 micron)

PDB DOI: https://doi.org/10.2210/pdb8RSD/pdb

Classification: SUGAR BINDING PROTEIN
Organism(s): Thaumatococcus daniellii
Mutation(s): No

Deposited: 2024-01-24 Released: 2024-12-04
Deposition Author(s): Bosman, R., Prester, A., von Soosten, L., Dibenedetto, S., Bartels, K., Sung, S., von Stetten, D., Mehrabi, P., Schulz, E.C.
Funding Organization(s): German Research Foundation (DFG), German Federal Ministry for Economic Affairs and Energy

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.70 Å
R-Value Free: 0.213
R-Value Work: 0.184
R-Value Observed: 0.185

Starting Model: experimental
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wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

Kapton-based HARE chips for fixed-target serial crystallography

Bosman, R., Prester, A., von Soosten, L., Dibenedetto, S., Bartels, K., Sung, S., von Stetten, D., Mehrabi, P., Blatter, G., Lu, G., Bernhard, S., Osbild, M., Schulz, E.C.

To be published.

Macromolecule Content

Total Structure Weight: 25.63 kDa
Atom Count: 1,789
Modelled Residue Count: 207
Deposited Residue Count: 235
Unique protein chains: 1

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Thaumatin I	A [auth X]	235	Thaumatococcus daniellii	Mutation(s): 0
UniProt
Find proteins for P02883 (Thaumatococcus daniellii) Explore P02883 Go to UniProtKB: P02883
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P02883
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
TAR Query on TAR Download Ideal Coordinates CCD File SDF format, chain B [auth X] MOL2 format, chain B [auth X] mmCIF format, chain B [auth X]	B [auth X]	D(-)-TARTARIC ACID C₄ H₆ O₆ FEWJPZIEWOKRBE-LWMBPPNESA-N		Interactions Focus chain B [auth X] Interactions & Density Focus chain B [auth X]

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.70 Å
R-Value Free: 0.213
R-Value Work: 0.184
R-Value Observed: 0.185
Space Group: P 4₁ 2₁ 2

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 58.2	α = 90
b = 58.2	β = 90
c = 150.9	γ = 90

Software Package:

Software Name	Purpose
PHENIX	refinement
CrystFEL	data reduction
CrystFEL	data scaling
PHASER	phasing

Structure Validation

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Entry History & Funding Information

Deposition Data

Released Date: 2024-12-04

Deposition Author(s):

Funding Organization	Location	Grant Number
German Research Foundation (DFG)	Germany	458246365
German Research Foundation (DFG)	Germany	451079909
German Federal Ministry for Economic Affairs and Energy	Germany	01KI2114

Revision History (Full details and data files)

Version 1.0: 2024-12-04
Type: Initial release

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8RSD

Thaumatin measured via serial crystallography from a kapton HARE-chip (125 micron)

Kapton-based HARE chips for fixed-target serial crystallography

Entity ID: 1

UniProt

Entity Groups

Sequence Annotations

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Experimental Data

Structure Validation

Deposition Data

Revision History (Full details and data files)