Download MendeleyDesign of an actin-severing peptide.
Scipion, C.P.M., Robinson, R.C.To be published.
6JH9
Crystal structure of an actin monomer in complex with a chimeric peptide of Cordon-Bleu WH2 mutant and MIM. Lys18Arg
- PDB DOI: https://doi.org/10.2210/pdb6JH9/pdb
- Classification: STRUCTURAL PROTEIN/PROTEIN BINDING
- Organism(s): Oryctolagus cuniculus, Mus musculus
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2019-02-17 Released: 2020-02-19
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.74 Å
- R-Value Free: 0.206
- R-Value Work: 0.184
- R-Value Observed: 0.185
Starting Model: experimental
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This is version 1.1 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Actin, alpha skeletal muscle | 377 | Oryctolagus cuniculus | Mutation(s): 0 EC: 3.6.4 |  | |
UniProt | |||||
Find proteins for P68135 (Oryctolagus cuniculus) Explore P68135 Go to UniProtKB: P68135 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P68135 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Cordon-Bleu MIM chimeric peptide | 29 | Mus musculus | Mutation(s): 0 Gene Names: Cobl, Kiaa0633 |  | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for Q5NBX1 (Mus musculus) Explore Q5NBX1 Go to UniProtKB: Q5NBX1 | |||||
IMPC: MGI:105056 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q5NBX1 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| ATP Query on ATP | C [auth A] | ADENOSINE-5'-TRIPHOSPHATE C10 H16 N5 O13 P3 ZKHQWZAMYRWXGA-KQYNXXCUSA-N |  | ||
| GOL Query on GOL | F [auth A] | GLYCEROL C3 H8 O3 PEDCQBHIVMGVHV-UHFFFAOYSA-N |  | ||
| CA Query on CA | D [auth A], E [auth A] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| HIC Query on HIC | A | L-PEPTIDE LINKING | C7 H11 N3 O2 |  | HIS |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.74 Å
- R-Value Free: 0.206
- R-Value Work: 0.184
- R-Value Observed: 0.185
- Space Group: C 1 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 195.35 | α = 90 |
| b = 56.368 | β = 93.66 |
| c = 38.478 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| XDS | data reduction |
| XDS | data scaling |
| PHASER | phasing |
Entry History
Deposition Data
- Released Date: 2020-02-19 Deposition Author(s): Scipion, C.P.M., Robinson, R.C.
Revision History (Full details and data files)
- Version 1.0: 2020-02-19
Type: Initial release - Version 1.1: 2023-11-22
Changes: Data collection, Database references, Derived calculations, Refinement description
