6NMM

Ternary complex structure of the T130K mutant of ANT-4 with Neomycin, AMPCPP and Pyrophosphate

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
AMR Gene: CARD Annotation
AMR Gene Family: CARD Annotation
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d6nmma2	All alpha proteins	Four-helical up-and-down bundle	Nucleotidyltransferase substrate binding subunit/domain	Kanamycin nucleotidyltransferase (KNTase), C-terminal domain	automated matches	Bacillus stearothermophilus (Geobacillus stearothermophilus ) [TaxId: 1422 ],	SCOPe (2.08)
A	d6nmma1	Alpha and beta proteins (a+b)	Nucleotidyltransferase	Nucleotidyltransferase	Kanamycin nucleotidyltransferase (KNTase), N-terminal domain	automated matches	Bacillus stearothermophilus (Geobacillus stearothermophilus ) [TaxId: 1422 ],	SCOPe (2.08)
B	d6nmmb2	All alpha proteins	Four-helical up-and-down bundle	Nucleotidyltransferase substrate binding subunit/domain	Kanamycin nucleotidyltransferase (KNTase), C-terminal domain	automated matches	Bacillus stearothermophilus (Geobacillus stearothermophilus ) [TaxId: 1422 ],	SCOPe (2.08)
B	d6nmmb1	Alpha and beta proteins (a+b)	Nucleotidyltransferase	Nucleotidyltransferase	Kanamycin nucleotidyltransferase (KNTase), N-terminal domain	automated matches	Bacillus stearothermophilus (Geobacillus stearothermophilus ) [TaxId: 1422 ],	SCOPe (2.08)
C	d6nmmc2	All alpha proteins	Four-helical up-and-down bundle	Nucleotidyltransferase substrate binding subunit/domain	Kanamycin nucleotidyltransferase (KNTase), C-terminal domain	automated matches	Bacillus stearothermophilus (Geobacillus stearothermophilus ) [TaxId: 1422 ],	SCOPe (2.08)
C	d6nmmc1	Alpha and beta proteins (a+b)	Nucleotidyltransferase	Nucleotidyltransferase	Kanamycin nucleotidyltransferase (KNTase), N-terminal domain	automated matches	Bacillus stearothermophilus (Geobacillus stearothermophilus ) [TaxId: 1422 ],	SCOPe (2.08)
D	d6nmmd2	All alpha proteins	Four-helical up-and-down bundle	Nucleotidyltransferase substrate binding subunit/domain	Kanamycin nucleotidyltransferase (KNTase), C-terminal domain	automated matches	Bacillus stearothermophilus (Geobacillus stearothermophilus ) [TaxId: 1422 ],	SCOPe (2.08)
D	d6nmmd1	Alpha and beta proteins (a+b)	Nucleotidyltransferase	Nucleotidyltransferase	Kanamycin nucleotidyltransferase (KNTase), N-terminal domain	automated matches	Bacillus stearothermophilus (Geobacillus stearothermophilus ) [TaxId: 1422 ],	SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	KNTase_C	e6nmmA2	A: alpha bundles	X: Four-helical up-and-down bundle	H: HEPN	T: Nucleotidyltransferase substrate binding subunit/domain	F: KNTase_C	ECOD (1.6)
A	NTP_transf_2	e6nmmA1	A: a+b three layers	X: Nucleotidyltransferase-like	H: Nucleotidyltransferase (From Topology)	T: Nucleotidyltransferase	F: NTP_transf_2	ECOD (1.6)
B	KNTase_C	e6nmmB2	A: alpha bundles	X: Four-helical up-and-down bundle	H: HEPN	T: Nucleotidyltransferase substrate binding subunit/domain	F: KNTase_C	ECOD (1.6)
B	NTP_transf_2	e6nmmB1	A: a+b three layers	X: Nucleotidyltransferase-like	H: Nucleotidyltransferase (From Topology)	T: Nucleotidyltransferase	F: NTP_transf_2	ECOD (1.6)
C	KNTase_C	e6nmmC1	A: alpha bundles	X: Four-helical up-and-down bundle	H: HEPN	T: Nucleotidyltransferase substrate binding subunit/domain	F: KNTase_C	ECOD (1.6)
C	NTP_transf_2	e6nmmC2	A: a+b three layers	X: Nucleotidyltransferase-like	H: Nucleotidyltransferase (From Topology)	T: Nucleotidyltransferase	F: NTP_transf_2	ECOD (1.6)
D	KNTase_C	e6nmmD2	A: alpha bundles	X: Four-helical up-and-down bundle	H: HEPN	T: Nucleotidyltransferase substrate binding subunit/domain	F: KNTase_C	ECOD (1.6)
D	NTP_transf_2	e6nmmD1	A: a+b three layers	X: Nucleotidyltransferase-like	H: Nucleotidyltransferase (From Topology)	T: Nucleotidyltransferase	F: NTP_transf_2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.460.10	Alpha Beta	2-Layer Sandwich	Beta Polymerase	domain 2	CATH (utative)
A	1.20.120.330	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	Nucleotidyltransferases domain 2	CATH (utative)
B	3.30.460.10	Alpha Beta	2-Layer Sandwich	Beta Polymerase	domain 2	CATH (utative)
B	1.20.120.330	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	Nucleotidyltransferases domain 2	CATH (utative)
C	3.30.460.10	Alpha Beta	2-Layer Sandwich	Beta Polymerase	domain 2	CATH (utative)
C	1.20.120.330	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	Nucleotidyltransferases domain 2	CATH (utative)
D	3.30.460.10	Alpha Beta	2-Layer Sandwich	Beta Polymerase	domain 2	CATH (utative)
D	1.20.120.330	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	Nucleotidyltransferases domain 2	CATH (utative)

AMR Gene: CARD Annotation CARD Database Homepage

Chains	Accession	Short Name	Description	Provenance Source (Version)
A, B, C, D	ARO:3002623	ANT(4')-Ia	ANT(4')-Ia is a plasmid-encoded aminoglycoside nucleotidyltransferase in S. epidermidis, S. aureus, Enterococcus spp. and Bacillus spp.	CARD (3.3.0)

AMR Gene Family: CARD Annotation CARD Database Homepage

Chains	Accession	Gene Family	Drug Class	Resistance Machanism	Provenance Source (Version)
A, B, C, D	ARO:3000229	ANT(4')	aminoglycoside antibiotic	antibiotic inactivation	CARD (3.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF07827	KNTase C-terminal domain (KNTase_C)	KNTase C-terminal domain	Kanamycin nucleotidyltransferase (KNTase) is involved in conferring resistance to aminoglycoside antibiotics and catalyses the transfer of a nucleoside monophosphate group from a nucleotide to kanamycin. This enzyme is dimeric with each subunit be ...	Kanamycin nucleotidyltransferase (KNTase) is involved in conferring resistance to aminoglycoside antibiotics and catalyses the transfer of a nucleoside monophosphate group from a nucleotide to kanamycin. This enzyme is dimeric with each subunit being composed of two domains. The C-terminal domain contains five alpha helices, four of which are organised into an up-and-down alpha helical bundle. Residues found in this domain may contribute to this enzyme's active site [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Kanamycin nucleotidyltransferase	transferase activity nucleotidyltransferase activity catalytic activity transferase activity, transferring phosphorus-containing groups binding nucleoside phosphate binding organic cyclic compound binding nucleotide binding heterocyclic compound binding small molecule binding cation binding ion binding metal ion binding	response to stimulus response to chemical response to antibiotic	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR043519	Nucleotidyltransferase superfamily	Homologous Superfamily
A, B, C, D	IPR012481	Kanamycin nucleotidyltransferase, C-terminal	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.