2AS0 | pdb_00002as0

Crystal Structure of PH1915 (APC 5817): A Hypothetical RNA Methyltransferase

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2as0a1	All beta proteins	PUA domain-like	PUA domain-like	Hypothetical RNA methyltransferase domain (HRMD)	Hypothetical protein PH1915, N-terminal domain	(Pyrococcus horikoshii ) [TaxId: 53953 ],	SCOPe (2.08)
A	d2as0a2	Alpha and beta proteins (a/b)	S-adenosyl-L-methionine-dependent methyltransferases	S-adenosyl-L-methionine-dependent methyltransferases	hypothetical RNA methyltransferase	Hypothetical protein PH1915, middle and C-terminal domains	(Pyrococcus horikoshii ) [TaxId: 53953 ],	SCOPe (2.08)
B	d2as0b1	All beta proteins	PUA domain-like	PUA domain-like	Hypothetical RNA methyltransferase domain (HRMD)	Hypothetical protein PH1915, N-terminal domain	(Pyrococcus horikoshii ) [TaxId: 53953 ],	SCOPe (2.08)
B	d2as0b2	Alpha and beta proteins (a/b)	S-adenosyl-L-methionine-dependent methyltransferases	S-adenosyl-L-methionine-dependent methyltransferases	hypothetical RNA methyltransferase	Hypothetical protein PH1915, middle and C-terminal domains	(Pyrococcus horikoshii ) [TaxId: 53953 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Hypothetical RNA methyltransferase domain (HRMD)	8024936	4002230	SCOP2 (2022-06-29)
A	SCOP2 Family	hypothetical RNA methyltransferase	8024937	4003167	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	PUA domain-like	8037315	3001015	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	S-adenosyl-L-methionine-dependent methyltransferases	8037316	3000118	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	S-adenosyl-L-methionine-dependent methyltransferases	8037316	3000118	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	PUA domain-like	8037315	3001015	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PUA_1	e2as0A1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: PUA domain	F: PUA_1	ECOD (1.6)
A	Methyltransf_11_2	e2as0A2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_11_2	ECOD (1.6)
B	PUA_1	e2as0B1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: PUA domain	F: PUA_1	ECOD (1.6)
B	Methyltransf_11_2	e2as0B2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_11_2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.30.130.10	Mainly Beta	Roll	Archaeosine Trna-guanine Transglycosylase	Chain: A, domain 4	CATH (4.3.0)
A	3.30.750.80	Alpha Beta	2-Layer Sandwich	Transcription Regulator spoIIAA	RNA methyltransferase domain (HRMD) like	CATH (4.3.0)
A	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)
B	2.30.130.10	Mainly Beta	Roll	Archaeosine Trna-guanine Transglycosylase	Chain: A, domain 4	CATH (4.3.0)
B	3.30.750.80	Alpha Beta	2-Layer Sandwich	Transcription Regulator spoIIAA	RNA methyltransferase domain (HRMD) like	CATH (4.3.0)
B	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF10672	S-adenosylmethionine-dependent methyltransferase (Methyltrans_SAM)	S-adenosylmethionine-dependent methyltransferase	-	Family
A, B	PF17785	PUA-like domain (PUA_3)	PUA-like domain	This PUA-like domain is found at the N-terminus of SAM-dependent methyltransferases [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	hypothetical protein PH1915	transferase activity, transferring one-carbon groups transferase activity methyltransferase activity catalytic activity RNA binding binding nucleic acid binding	methylation metabolic process cellular process ribonucleoprotein complex biogenesis RNA metabolic process primary metabolic process rRNA processing gene expression nucleobase-containing compound biosynthetic process macromolecule biosynthetic process nucleic acid metabolic process nucleobase-containing compound metabolic process cellular component biogenesis RNA biosynthetic process methylation metabolic process cellular process ribonucleoprotein complex biogenesis RNA metabolic process primary metabolic process rRNA processing gene expression nucleobase-containing compound biosynthetic process macromolecule biosynthetic process nucleic acid metabolic process nucleobase-containing compound metabolic process cellular component biogenesis RNA biosynthetic process ribosome biogenesis nucleic acid biosynthetic process biosynthetic process macromolecule metabolic process cellular component organization or biogenesis RNA processing rRNA metabolic process	cellular anatomical structure intracellular anatomical structure cytoplasm

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR015947	PUA-like superfamily	Homologous Superfamily
A, B	IPR002478	PUA domain	Domain
A, B	IPR019614	S-adenosylmethionine-dependent methyltransferase	Domain
A, B	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase superfamily	Homologous Superfamily
A, B	IPR036974	PUA domain superfamily	Homologous Superfamily
A, B	IPR041532	RlmI-like, PUA domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.